KMID : 0380020120270020127
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Korean Journal of Biotechnology and Bioengineering 2012 Volume.27 No. 2 p.127 ~ p.130
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Optimization of gamma-Aminobutyric Acid Bioconversion by Recombinant Escherichia coli
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Vo Tam Dinh Le
Hong Soon-Ho
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Abstract
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In this study, the effects of pH, temperature, IPTG concentration and substrate (MSG) concentration on gamma-aminobutyric acid (GABA) production in engineered Escherichia coli were investigated. Glutamate decarboxylase and glutamate/GABA antiporter were overexpressed in GABA aminotransferase knock-out strain for GABA production. The result of optimization study showed the GABA bioconversion was optimized at pH 3.5, 30oC, 0.5 mM IPTG, 10 g/L MSG. At this condition, 5.23 g/L of final GABA concentration of was achieved from 10 g/L of MSG, which corresponded to a GABA yield of 85.77%.
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KEYWORD
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gamma-Aminobutyric acid, Escherichia coli, Bioconversion, Optimization
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